Title -Scientist I/II, Gene Transfer Technologies -About the Organization -REGENXBIO is a leading clinical-stage biotechnology company seeking to improve lives through the curative potential of gene therapy. REGENXBIO's NAV® Technology Platform, a proprietary adeno-associated virus (AAV) gene delivery platform, consists of exclusive rights to more than 100 novel AAV vectors, including AAV7, AAV8, AAV9 and AAVrh10. REGENXBIO and its third-party NAV Technology Platform Licensees are applying the NAV Technology Platform in the development of a broad pipeline of candidates in multiple therapeutic areas. Our gene therapy product candidates are designed to deliver genes to cells to address genetic defects or to enable cells in the body to produce therapeutic proteins that are intended to impact disease. Through a single administration, our gene therapy product candidates are designed to provide long-lasting effects, potentially significantly altering the course of disease and delivering improved patient outcomes. We are currently developing gene therapy product candidates for the treatment of retinal, metabolic, and neurodegenerative diseases. Our product candidates all utilize AAV viral vectors from our proprietary gene delivery platform, which we call our NAV Technology Platform. In addition to our internal product candidate programs, we also selectively license our NAV Vectors to other leading biotechnology companies. -Req Number -RE-18-00012 -Description -POSITION SUMMARY Under direction of the Sr. Scientist, the Scientist I/II will be responsible for creation of novel AAV vectors, as well as development and implementation cell based assays to evaluate AAVs. This position requires significant experience in directed evolution and synthetic biology, specifically towards construction and screening of large libraries. The incumbent will collaborate with other research groups as well as process development and manufacturing to advance novel AAV therapeutics. Extensive and current hands-on experience in cloning, nucleic acid purification, qPCR, site directed mutagenesis, and DNA sequence analysis is required. Work experience using viral vectors such as AAV, adenovirus or lenti virus, cell based assays, and primary cell culture is desired. Good communication and documentation skills are essential. PRIMARY RESPONSIBILITIES -Collaborate with the R&D team to develop and test new AAV vectors. -Clone, express and purify proteins, and viral capsids. -Develop new methods and assays. -Assist in lab management tasks, such as material inventory, ordering and equipment maintenance. -Maintain impeccable records from raw data including data books and computer generated data files, quantitative analysis of data, laboratory protocols, and summary reports. -Present data to small working groups as well as to the larger project teams. -Position Requirements -SKILLS, KNOWLEDGE AND ABILITIES REQUIRED -Ph.D. with 1-3 years of post-PhD experience in research and development of gene therapy products, or 2-4+ years of research and development in biologics, pharmaceutical or vaccine products. -Expertise in protein and/or antibody engineering. -Demonstrated ability in directed evolution and construction and screening large mutant libraries. -Working knowledge of NGS techniques and applications. -Experience with primary cell culture and/or tissue models. -Specific hands on skills and familiarity with molecular biology and protein expression techniques such as cloning, PCR, sequence analysis, and transient transfection. -Excellent oral and written communication skills for effectively interfacing with internal and external organizations. -Detailed oriented, organized, technically competent and be able to perform hands on work in the laboratory. WORKING CONDITIONS AND/OR PHYSICAL REQUIREMENTS -Sitting or standing for extended periods -Lifting to 30 lbs -Travel up to 20% MANAGER/SUPERVISORY RESPONSIBILITIES None. -Location -Rockville, Maryland -Exempt/Non-Exempt -Exempt -EOE Statement -We are an equal employment opportunity employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, gender, national origin, disability status, protected veteran status or any other characteristic protected by law. -Open Date -1/2/2019 -This position is currently accepting applications.