Job Description

To ensure that our cell analysis products deliver outstanding performance and exceed our customers’ expectations, BD Biosciences is seeking a passionate and capable Systems Validation Scientist II to contribute to the R&D team by preparing samples for flow cytometric studies. MUST HAVES ON THE RESUME:

1. Best practice experience doing antibody methods for antigen labeling (e.g., flow cytometry, western blots, ELISA, ELISPOT)
2. Experience working in a lab, with basic lab skills and knowledge (pipetting, dilutions, titrations, etc.)
3. Ability to purify leukocytes from mammalian-derived samples (i.e., venous blood) via different density-gradient centrifugation methods and RBC lysis protocols.
4. Ability to stain cells in tubes and plates for intracellular and extracellular antigens. Responsibilities: The Systems Validation Scientist II will play a critical role in preparing samples for flow cytometric studies. RESPONSIBLE FOR: • Maintaining the health, expansion, and inventory of cell lines • Performing cryopreservation and thawing of mammalian cells • Purification of leukocytes from mammalian-derived samples (i.e., venous blood) via different density-gradient centrifugation methods and RBC lysis protocols. • Staining of cells in tubes and plates for intracellular and extracellular antigens. • Fixation and permeabilization of cells, treatment of cells in culture with various agents, maintaining documents/lot numbers of antibodies and reagents. • Counting cells via hemocytometer and/or proprietary cell counters. • Preparing buffers, media, and other reagents. • Maintaining inventory of critical laboratory supplies (consumables and reagents) in the lab • Attend meetings, as needed. Qualifications: The successful candidate will possess: • A minimum of a bachelor’s degree in Microbiology, Biology, Biochemistry, Immunology, or a related field. • At least 1-2 years of experience working in a Biosafety Level 2 laboratory environment, performing cell culture with multiple different mammalian cell lines. • Best practice experience doing antibody methods for antigen labeling (e.g., flow cytometry, western blots, ELISA, ELISPOT) • Experience working with primary cells derived from various mammalian species. • Proficient with phase-contrast and brightfield microscopy. • Excellent aseptic technique. • Ability to troubleshoot common technical problems associated with cell sample preparation for flow cytometric studies. • Ability to calculate basic laboratory calculations. • Excellent attention to detail, can work quickly and accurately, organizing tasks and delivering quality with efficiency. • Ability to read and understand Standard Operating Procedures • Ability to interact with researchers for delivery of cells. • Ability to maintain a clean work area. • Knowledge of GLP/GDP guidelines. • Experience working in a lab, with basic lab skills and knowledge (pipetting, dilutions, titrations, etc.) • Excellent written and oral communication skills.