Job Description

BECOME YOUR MOST EXTRAORDINARY SELF!  OUR TEAM IS INTENSELY PROUD TO BE A MAJOR PROVIDER OF SERVICES AND PRODUCTS TO THE DRUG DISCOVERY RESEARCH INDUSTRY.  JOIN EUROFINS DISCOVERX PRODUCTS, LLC PART OF THE EUROFINS DISCOVERY ™ GROUP OF COMPANIES SERVING GLOBAL CLIENTS IN THE PHARMA AND BIOTECH INDUSTRIES.  WORK ALONGSIDE INDUSTRY EXPERTS AND COLLABORATE WITH TEAM MEMBERS TO DIRECTLY AND POSITIVELY IMPACT HUMAN HEALTH WORLDWIDE!

WE HAVE A PHILOSOPHY TO SUPPORT WORK LIFE BALANCE, CAREER PROGRESSION OPPORTUNITIES AND OFFER MANY BENEFITS SUCH AS FORMAL MENTORING PROGRAMS, TUITION REIMBURSEMENT, EXTENSIVE CAREER TRAINING PROGRAMS, COMPETITIVE HEALTH BENEFITS, PAID TIME OFF AND 10 ANNUAL PAID HOLIDAYS*.  

ESSENTIAL DUTIES AND RESPONSIBILITIES:

• Generate expression plasmids required for all mammalian cell-based assay development activities at Eurofins DiscoverX.
• Routinely submit samples to, and evaluate data returned by, our external DNA sequencing partner
• Perform PCR on genomic DNA from cell lines to confirm stable integration of genes and quantitative PCR to confirm gene expression
• Actively maintain the Eurofins DiscoverX plasmid collection including fulfilling requests for plasmids and generating additional quantities of plasmids by performing DNA preps (in-house or via out-sourcing)
• Use bioinformatics tools (including SnapGene and NCBI databases etc) for executing and confirming cloning projects.
• Design DNA constructs: identify the correct gene sequence (according to the workplan); optimize the DNA sequence and confirm in-frame fusion with tags (when applicable).
• Perform RNA extraction and quantify gene expression by qRT-PCR
• Create first draft of Molecular Biology Milestone Report (MS1)
• Generate Biosafety Documentation
• Serve as backup for staff primarily responsible to generate retrovirus and lentiviruses for mammalian gene delivery in vitro.
• Ability to multitask and communicate effectively to ensure orderly completion and transfer of plasmids to downstream assay development projects.
• Maintain good documentation of cloning and laboratory work using various internal databases and electronic lab notebook.
• Participate in weekly departmental lab meeting.

Qualifications

• Minimum of 1-3 years as a molecular biology contributor is required.
• Knowledge and demonstrated use of basic methods for molecular biology (restriction enzymes, subcloning, agarose gels, analysis of DNA sequencing, minipreps, PCR)
• Bachelor’s degree or equivalent experience in molecular biology, cell biology, biochemistry or related discipline.
• Experience planning and troubleshooting subcloning projects is preferred.
• Experience in quantitative PCR (qPCR) and reverse transcriptase-PCR (qRT-PCR) is desirable.
• Work with various modes of gene delivery (virus, lipid & electroporation) including retroviral or lentiviral vector systems is desirable.
• Industry experience is preferred.
• Experience with mammalian cell culture and cell-based assays while working under BSL1/2 is desirable.
• Skills in any or all of the following are desirable: Western blotting, immunofluorescence microscopy and Flow cytometry.

Additional Information

• Excellent full time benefits including comprehensive medical coverage, dental, and vision options
• Life and disability insurance
• 401(k) with company match
• Paid vacation and holidays

EUROFINS IS A M/F, DISABLED, AND VETERAN EQUAL EMPLOYMENT OPPORTUNITY AND AFFIRMATIVE ACTION EMPLOYER.