Job Description
This is an exciting time to join the Eurofins Genomics LLC. The company is rapidly expanding oligonucleotide manufacturing to support the increasing demand of molecular diagnostics. You will work directly with some of the biggest companies in life sciences to manufacture and develop oligonucleotides (oligos) and support ever evolving needs. Eurofins Genomics LLC has been active in supporting pandemic relief with primers/probe production for SARS-CoV-02 in addition to being a key supplier of oligos for other diagnostic applications. We have also teamed up with our local community to support projects in SARS-CoV-02 community surveillance.
The Nucleic Acid Chemist will be an owner in area of expertise (oligonucleotide synthesis, deprotection, purification, analysis and quality control). This position reports directly to the Principal Scientist.
General Overall Responsibilities:
- Independently develop and execute experimental plans with minimal supervision
- Manage the area priorities based on aligned company needs
- Develop initial plan for customer projects in area of expertise
- Innovate and introduce automation in order to reduce waste and improve efficiencies
- Work with vendors to develop best practices for equipment in area
- Responsible for IQ, OQ and PQ for synthesis, downstream manufacturing, and analytical equipment
- Automate workflows in area to improve lead times and mitigate manual errors
- Write SOPs and equipment plans. Train lab personnel. Maintain QMS documents as current.
- Work with Environmental Health and Safety, EHS, to ensure area fully conforms to highest safety standards
- Conduct QMS / EHS audits and complete assigned improvement actions
** Synthesis Specific**
- Serve as subject matter expert for large scale AKTA, BLP, and Internal Synthesis Platforms
- Monitoring and management of current synthesis machines to ensure operation at basic condition and detect deviations from basic condition
- Development of new protocols that include new 3’, internal, and 5’ modifications within synthesis process
- Development of new synthesis scales, frit development, plate loading automation, and synthesis column fill automation
- Lead qualification of new materials including CPG, Amidites, and Reagents
- Work with operations team for new projects to develop synthesis strategy
- Lead cost reduction projects through reduction of reagents, maximization of yields
** Liquid Handling and Automation Specific**
- Method development for oligo normalization, quantification using liquid handing equipment such as Hamilton Microlabs, Echo, TECANs etc.
- Ability to develop new methods for deprotection/cleavage processes to suit various oligo modifications
- Develop criteria that ensures liquid handling equipment maintains specification with triggered action on drift from basic condition
- Train lab personnel on developed methods and transfer methods for high throughput and specialized production
** Purification Specific**
- HPLC method development for specific DNA/RNA modifications, scales, lengths, and purity
- Customization of method and mobile phases to maximize yields while maintaining quality
- Develop new downstream workflows for automated larger scale purification process
- Develop and implement other purification processes (PAGE, Cartridge, etc) that expand portfolio for purification