Job Description

Rejuvenate Bio is looking for a highly motivated individual to join our energetic team and accelerate the development of our therapies. The position will directly support efforts to drive forward our future commercial offerings by developing our GMP process. The Bioprocess Engineer will be responsible for independently developing and optimizing mammalian cell culture processes for the production of AAV-based gene therapies. This role reports directly to the Sr. Director of Process Development and will work alongside a team of Associates on upstream process development, scale-up, optimization, characterization, and transfer of projects.

RESPONSIBILITIES:

• Independently develop, design, and conduct viral vector production experiments and evaluate new technologies to increase AAV vector yield and process understanding using design of experiment (DOE)
• Performing cell culture (adherent and suspension) for virus creation and purification
• Using statistical approaches (DOE, QbD) for process optimization at small scale and translating results to bioreactor scale production.
• Performing researching into ways of enhancing AAV production
• Leads, plans and conducts viral vector production in bioreactors ranging from 1L to 50L scale
• Performing standard and advanced cloning techniques
• Executing experiments, independently and in conjunction with a small team in support of research and clinical applications
• Following laboratory protocols and safety regulations
• Performing detailed and accurate documentation of all experiments and laboratory practices
• Accountable for on-time, high quality results and clearly communicating progress
• Perform all other duties as assigned by supervisor or manager

Qualifications

BASIC QUALIFICATIONS:

• B.S. or M.S. in virology, biology, biochemistry, microbiology, biomedical engineering, bioengineering or related field required.
• 5+ years of experience optimizing mammalian cell culture processes for production of biological products, AAV vectors preferred.
• Hands on experience in upstream bioprocess unit operations including shaker flasks, stirred tank bioreactors (e.g. AMBR15/250, Das Gip, or similar) and a desire to continue in a laboratory- focused role.
• Experience with large scale transfections. 
• Extensive understanding of analytical tools and calculations such as cell counting, oxygen uptake rates, metabolite consumption rates, viral vector titer and product quality assessments.

PREFERRED QUALIFICATIONS:

• 2+ years of experience with AAV vectors preferred.
• Experience with suspension cell culture and AAV production.
• Experience with flow cytometry, ELISAs, PCR, and microscopy.
• Proven ability to draft and implement lab protocols.
• Ability to work independently as well as in a collaborative team environment.
• Strong work ethic, communication skills and goal-oriented personality.

Additional Information

BASE SALARY: $80k - $120k+; exact salary and job title will depend on experience.

PHYSICAL REQUIREMENTS: The physical demands described here are representative of those that must be met by an employee to successfully perform the essential functions of this job. Reasonable accommodations may be made to enable individuals with disabilities to perform the essential functions.

• Position is primarily sedentary, and employee may be required to sit for extended periods of time.
• Standing, walking and meeting activities are required frequently throughout the workday.
• Must be able to process paperwork and utilize office equipment (including personal computer, phone, copiers, etc.).
• Normal sight or corrected vision is required to read documents and use standard computer terminals. Some light lifting may be occasionally required.
• Ability to safely and successfully perform the essential job functions consistent with the ADA, FMLA and other federal, state and local standards, including meeting qualitative and/or quantitative productivity standards.
• Ability to maintain regular, punctual attendance consistent with the ADA, FMLA and other federal, state and local standards
• Must be able to lift and carry up to 25 lbs
• Must be able to talk, listen and speak clearly on telephone

EEO STATEMENT Rejuvenate Bio is committed to equal employment opportunity and non-discrimination for all employees and qualified applicants without regard to a person's race, color, sex, gender identity or expression, age, religion, national origin, ancestry, ethnicity, disability, veteran status, genetic information, sexual orientation, marital status, or any characteristic protected under applicable law. Rejuvenate Bio is an E-Verify Employer in the United States. All your information will be kept confidential according to EEO guidelines.

THE HEALTH AND SAFETY OF OUR EMPLOYEES AND THEIR FAMILIES IS A TOP PRIORITY. The company encourages employees to remain up-to-date on their COVID-19 vaccinations. Rejuvenate Bio, Inc. employees may be required, in the future, to be vaccinated or have an approved disability/medical or religious accommodation.