We are looking for an experienced lab-based Associate Scientist / Senior Associate Scientist with expertise in molecular biology and assay development to join our team. In this role you will embrace the hands-on ethos of a start-up company, provide critical support for combinatorial cloning efforts, and generate in vitro data to validate our therapeutic platform.

Our work is team-based, and this role requires extensive collaboration with colleagues in therapeutic and platform development teams. If you are intellectually curious and you take a data-driven and rigorous approach to solving problems – this role may be right for you.

YOUR RESPONSIBILITIES

In this role, you will be responsible for the design, execution, troubleshooting, and optimization of molecular biology assays, including:

Generating and maintaining DNA construct libraries for test article production.

Performing high throughput cloning of plasmid constructs with Golden Gate assembly and conventional cloning and oversee sequence verification by external vendors.

Leading Next Generation Sequencing (NGS) library preparation for RNA-seq, DNA-seq, 10x Genomics single-cell assays.

Screening of high-throughput plasmid and viral libraries in mammalian lines and primary cell cultures.

Facilitate design of plasmid constructs using DNA software.

Organizing and tracking DNA constructs and associated sequencing data across multiple projects.

Collaboration with teammates to execute project plans, present data and recommend next steps.

Authoring research reports and regulatory documents to support first-in-human studies and contribute to intellectual property filings.

Documenting research activities and findings in electronic laboratory notebook and reagent registries in keeping with the highest industry standards.

YOUR PRIOR EXPERIENCE

Gibson Assembly, Gateway and conventional cloning methods.

Molecular biology software such as SnapGene or Geneious.

Plasmid construct design, agarose gel electrophoresis, DNA purification, plasmid preparation, primer design, and DNA sequence analysis.

Next Generation Sequencing (NGS) library preparation for RNA-seq, DNA-seq, 10x Genomics assays.

Plasmid transfection of cultured mammalian cells.

Genomic DNA and RNA purification from various mammalian cell lines and primary cells.

Molecular assays such as RT-PCR, digital PCR, and ELISA.

Transduction of mammalian cells with AAV and/or lentivirus.

Multicolor flow cytometry, FACS and fluorescence microscopy.

YOUR QUALIFICATIONS

BS or equivalent degree in biology, biochemistry, bioengineering, or a related discipline

1-4 years of relevant industry experience

Passion for organization and experience with sample tracking and maintenance of reagent registries

Excellent analytical, organizational, communication, and time management skills.

Zag Bio values the diversity of our workforce and maintains a deeply respectful, values-driven working environment. We are committed to equal employment opportunities and non-discrimination for all employees and qualified applicants without regard to a person's race, color, gender, age, religion, national origin, ancestry, disability, veteran status, genetic information, sexual orientation, or any characteristic protected under applicable law.

We are not accepting unsolicited resumes from employment agencies for any employment opportunities at this time.

Interested candidates should e-mail their CV including a brief description of interest to HR@zagbio.com